Cow manure, commonly used to fertilize vegetable crops,contains a high number of genes that can fuel resistance to antibiotics, a US studyout Tuesday found.These genes come from the cows' gut bacteria, and while none have yet been foundin superbugs that are infecting humans, researchers said the potential is real. The research was done by scientists at Yale University, who sampled manure from ahandful of dairy cows at a farm in Connecticut.In those samples, they found 80 unique antibiotic resistance genes.About three quarters were unfamiliar. Genetic sequencing showed they were onlydistantly related to those already known to science.When applied to a lab strain of E. coli, the genes made the bacteria resistant tocertain well-known antibiotics, including penicillin and tetracycline.Researchers said they were surprised by the number of antibiotic resistance (AR) genes they found, based on just five stool samples from four cows.However, they also noted that the levels were lower than what is seen in chickens,which are often fed four times as many antibiotics as cows -- typically to promotegrowth."The diversity of genes we found is remarkable in itself considering the small set offive manure samples," said Jo Handelsman, senior study author and microbiologistat Yale."But also, these are evolutionarily distant from the genes we already have in thegenetic databases, which largely represent AR genes we see in the clinic."Further study is needed to probe whether cow manure may harbor a major reservoirof antibiotic resistance genes that could move into humans."This is just the first in a sequence of studies —- starting in the barn, moving to thesoil and food on the table and then ending up in the clinic -- to find out whetherthese genes have the potential to move in that direction," Handelsman said.The study appears in mBio, an open access online journal published by the AmericanSociety for Microbiology.Funding for the research came from the Swiss National Science Foundation and theUS National Institutes of Health.